In vitro assessment of respiratory toxicity: selection of a suitable cell line
With the human lung having more than 60 different cell types and a variety of different cell functions present, the in vitro assessment of respiratory toxicity is a challenging endeavor. Each cell type and the function it performs represents a potential target for a toxic mechanism of action. The first question when selecting a suitable […]
Intra- and Inter-assay variability of cytotoxicity testing
The viability of cells in cytotoxicity assays is always calculated relative to untreated control cells that undergo the same seeding and exposure procedure but receive only crude extraction media that has been incubated without the test item. Moreover, the spectrometric signal measured for a specific well in the assay is always corrected by blanks in the assay […]
Intralaboratory comparability of cytotoxicity testing conducted according to ISO 10993-5
In vitro cytotoxicity testing is an essential part of the biocompatibility assessment of new materials. However, the ISO 10993-5 standard allows a high degree of variability in the choice of test conditions between individual tests. It is therefore not always possible to directly compare test results between tests without taking the actual test conditions into account. In […]
Interlaboratory comparability of cytotoxicity testing conducted according to ISO 10993-5
The ISO standard for the performance of cytotoxicity assays for medical devices does not explicitly specify the type of cytotoxicity assay to be used and also allows a high degree of freedom in the choice of exact test parameters. This may lead to significant differences in test results among laboratories and depending on the type […]